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(A) Western blot analysis shows the membranous levels of PSD-95 in the hippocampus of young-adult rats receiving dexamethasone (Dex) at P7. PSD-95 levels were significantly higher in the Dex group (1.90 ± 0.11) than in the NS group (1.00 ± 0.07) (Mann-Whitney test, * p < 0.05). Tubulin was used as an internal control. The data are expressed as mean ± SEM of three independent experiments ( n = 6 for each group). Administration of dexamethasone (Dex) at P7 does not affect the membranous level of AMPA receptors in the hippocampus measured at P56. (B-E) The membranous level of AMPA receptor subunits, <t>GluR1</t> (B) and GluR2 (C), did not differ significantly between the Dex and NS groups. The expression levels of NR2A (D) and NR2B (E) were significantly higher in the Dex group (NR2A: 1.97 ± 0.20; NR2B: 2.21 ± 0.24) than in the NS group (NR2A: 1.00 ± 0.05; NR2B: 1.00 ± 0.08) (Mann-Whitney test, * p < 0.05). Tubulin was used as an internal control. These measurements were done after the contextual or cue-feared conditioning. The data are expressed as mean ± SEM of three independent experiments ( n = 6 each group).
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(A) Western blot analysis shows the membranous levels of PSD-95 in the hippocampus of young-adult rats receiving dexamethasone (Dex) at P7. PSD-95 levels were significantly higher in the Dex group (1.90 ± 0.11) than in the NS group (1.00 ± 0.07) (Mann-Whitney test, * p < 0.05). Tubulin was used as an internal control. The data are expressed as mean ± SEM of three independent experiments ( n = 6 for each group). Administration of dexamethasone (Dex) at P7 does not affect the membranous level of AMPA receptors in the hippocampus measured at P56. (B-E) The membranous level of AMPA receptor subunits, <t>GluR1</t> (B) and GluR2 (C), did not differ significantly between the Dex and NS groups. The expression levels of NR2A (D) and NR2B (E) were significantly higher in the Dex group (NR2A: 1.97 ± 0.20; NR2B: 2.21 ± 0.24) than in the NS group (NR2A: 1.00 ± 0.05; NR2B: 1.00 ± 0.08) (Mann-Whitney test, * p < 0.05). Tubulin was used as an internal control. These measurements were done after the contextual or cue-feared conditioning. The data are expressed as mean ± SEM of three independent experiments ( n = 6 each group).
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phospho-rb (ser807/811) alphalisa surefire ultra detection kit, 500 assay points  (PerkinElmer)
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The AlphaLISA® SureFire® Ultra™ p-Rb (Ser807/811) assay is a sandwich immunoassay for quantitative detection of phospho-Rb (phosphorylated on Ser807/811) in cellular lysates using Alpha Technology.
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Image Search Results


Journal: iScience

Article Title: Daughter cell fate choice instructed preemptively by mother cells facing nutrient limitation

doi: 10.1016/j.isci.2023.107198

Figure Lengend Snippet:

Article Snippet: phospho-Rb (Ser807/811) conjugated with Alexa Fluor® 488 , CST , 4277S.

Techniques: Recombinant, Negative Control, Software, Time-lapse Microscopy, Imaging

(A) Western blot analysis shows the membranous levels of PSD-95 in the hippocampus of young-adult rats receiving dexamethasone (Dex) at P7. PSD-95 levels were significantly higher in the Dex group (1.90 ± 0.11) than in the NS group (1.00 ± 0.07) (Mann-Whitney test, * p < 0.05). Tubulin was used as an internal control. The data are expressed as mean ± SEM of three independent experiments ( n = 6 for each group). Administration of dexamethasone (Dex) at P7 does not affect the membranous level of AMPA receptors in the hippocampus measured at P56. (B-E) The membranous level of AMPA receptor subunits, GluR1 (B) and GluR2 (C), did not differ significantly between the Dex and NS groups. The expression levels of NR2A (D) and NR2B (E) were significantly higher in the Dex group (NR2A: 1.97 ± 0.20; NR2B: 2.21 ± 0.24) than in the NS group (NR2A: 1.00 ± 0.05; NR2B: 1.00 ± 0.08) (Mann-Whitney test, * p < 0.05). Tubulin was used as an internal control. These measurements were done after the contextual or cue-feared conditioning. The data are expressed as mean ± SEM of three independent experiments ( n = 6 each group).

Journal: PLoS ONE

Article Title: A Single Postnatal Dose of Dexamethasone Enhances Memory of Rat Pups Later in Life

doi: 10.1371/journal.pone.0165752

Figure Lengend Snippet: (A) Western blot analysis shows the membranous levels of PSD-95 in the hippocampus of young-adult rats receiving dexamethasone (Dex) at P7. PSD-95 levels were significantly higher in the Dex group (1.90 ± 0.11) than in the NS group (1.00 ± 0.07) (Mann-Whitney test, * p < 0.05). Tubulin was used as an internal control. The data are expressed as mean ± SEM of three independent experiments ( n = 6 for each group). Administration of dexamethasone (Dex) at P7 does not affect the membranous level of AMPA receptors in the hippocampus measured at P56. (B-E) The membranous level of AMPA receptor subunits, GluR1 (B) and GluR2 (C), did not differ significantly between the Dex and NS groups. The expression levels of NR2A (D) and NR2B (E) were significantly higher in the Dex group (NR2A: 1.97 ± 0.20; NR2B: 2.21 ± 0.24) than in the NS group (NR2A: 1.00 ± 0.05; NR2B: 1.00 ± 0.08) (Mann-Whitney test, * p < 0.05). Tubulin was used as an internal control. These measurements were done after the contextual or cue-feared conditioning. The data are expressed as mean ± SEM of three independent experiments ( n = 6 each group).

Article Snippet: The following primary antibodies were used: mouse monoclonal GluR1 (1:1000; Santa Cruz Biotechnology, Santa Cruz, CA; SC-13152) and GluR2 (1:1000; Santa Cruz Biotechnology; SC-7610), goat polyclonal NR2A (1:2000; Santa Cruz Biotechnology; SC-1468), rabbit NR2B (1:5000; Cell Signaling Technology; No. D15B3), polyclonal rabbit PSD-95 (1:50000; Abcam, Cambridge, UK; ab18258), rabbit PI3Kp110 and PI3Kp85 (1:1000; Cell Signaling Technology; No. 4255s and 4292s, respectively), and rabbit NF-κBp65 (1:1000; Santa Cruz Biotechnology; SC-372).

Techniques: Western Blot, MANN-WHITNEY, Control, Expressing